the relaxant effects of different methanolic fractions of nigella sativa on guinea pig tracheal chains

Authors

rana keyhanmanesh tuberculosis and lung research centre, tabriz university of medical sciences, tabriz, iran

horeyeh bagban department of physiology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

hossein nazemiyeh research centre for pharmaceutical nanotechnology, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran

fariba mirzaei bavil department of physiology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

abstract

objective(s): in regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. our previous studies demonstrated the preventive effect of nigella sativa extract on ovalbumin-induced asthma. in addition, water-soluble substances of n. sativa extract and methanol fraction of this plant were responsible for the relaxant effect of this plant on tracheal chains of guinea pigs. therefore, for the first time, in the present study, in order to identify main constituents of the methanolic extract, the relaxant effects of five different methanolic fractions (20%, 40%, 60%, 80%, and 100%) of n. sativa   on tracheal chains of guinea pigs were examined.     materials and methods:     the relaxant effects of four cumulative concentrations of each fraction (0.8, 1.2, 1.6, and 2.0 g%) in comparison with saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6, and 0.8 mm) were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60 mm kcl (group 1) and 10 μm methacholine (group 2).     results: in group 1, all concentrations of only theophylline showed significant relaxant effects but all concentrations of these methanolic fractions showed significant contractile effects compared with that of saline (p<0.001 to p<0.05). however, in group 2, all concentrations of theophylline and these methanolic fractions showed significant relaxant effects compared with that of saline (p<0.001 to p<   0.05).     conclusion: these results showed a potent relaxant effect of 20% methanolic fractions from n. sativa   on tracheal chains of guinea pigs that were higher than that of theophylline at the used concentrations

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains

  Objective(s): In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant eff...

full text

The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains

OBJECTIVE(S) In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant effect...

full text

Relaxant effects of different fractions from Nigella sativa L. on guinea pig tracheal chains and its possible mechanism(s).

The relaxant effects of four cumulative concentrations of n-hexane, dichloromethane, methanol and aqueous fractions of N. sativa (0.8, 1.2, 1.6 and 2.0 g%) in comparison with saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mM) were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 ...

full text

the main relaxant constituents of nigella sativa methanolic fraction on guinea pig tracheal chains.

our  previous  studies demonstrated  the  preventive effect  of  nigella  sativa  extract  on asthma and water-soluble substances of this extract, especially its methanol fraction were responsible for this relaxation on contracted tracheal chains of guinea pigs. for the first time, the present study has been conducted to determine the main constituents of its methanolic extract. four constituen...

full text

Possible Mechanism(s) of the Relaxant Effects of Achillea wilhelmsii on Guinea-Pig Tracheal Chains

Achillea wilhelmsii have been used in folk remedies. The relaxant effects of the extractof A. wilhelmsii on tracheal chains of guinea pigs were examined. The relaxant effects offour cumulative concentrations of the extract, theophylline and saline were examined bytheir relaxant effects on precontracted tracheal chains of guinea pig by KCl (group 1), 10 μMmethacholine (group 2), incubated tissue...

full text

Possible Mechanism(s) of the Relaxant Effects of Achillea wilhelmsii on Guinea-Pig Tracheal Chains

Achillea wilhelmsii have been used in folk remedies. The relaxant effects of the extractof A. wilhelmsii on tracheal chains of guinea pigs were examined. The relaxant effects offour cumulative concentrations of the extract, theophylline and saline were examined bytheir relaxant effects on precontracted tracheal chains of guinea pig by KCl (group 1), 10 μMmethacholine (group 2), incubated tissue...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۶، شماره ۲، صفحات ۱۲۳-۱۲۸

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023