effect of mesenchymal stem cells on doxorubicin-induced fibrosis

Authors
abstract

objective: the aim of this study was to test the effect of intravenous injection of mesenchymal stem cells (mscs) on doxorubicin (dox)-induced fibrosis in the heart. we investigated the mechanisms that possibly mediate this effect. materials and methods: in this experimental study, fibrosis in the myocardium of adult male wistar rats (weights 180-200 g, 9-10 weeks of age, total n=30) was created by dox administration. dox (2.5 mg/kg) was administered intraperitoneally 3 times a week, for a total dose of 15 mg/kg over a period of 2 weeks. mscs from wistar rats were separated and cultured in dulbecco’s modified eagle medium (dmem). the condition medium (cm) which contained factors secreted by mscs was also collected from mscs cultured in serum-free dmem. two weeks after the first injection of dox, mscs, cm and standard medium (sm) were transplanted via intravenous injection. four weeks after transplantation, histological (masson’s trichrome staining for fibrosis detection) and molecular [real-time polymerase chain reaction (rt-pcr)] analyses were conducted. in addition, insulin-like growth factor (igf-1) and hepatocyte growth factor (hgf) in the cm were measured with an enzyme-linked immunosorbent assay (elisa). for immunosuppressive treatment, cyclosporine a was given (intraperitoneally, 5 mg/kg/day) starting on the day of surgery until the end of study in all groups. fibrosis rate and relative gene expression were compared by analysis of variance (anova) and post-tukey’s test. hgf and (igf-1 in the cm were analyzed by independent sample t test. p<0.01 was considered statistically significant. results: our data demonstrated that intravenously transplanted mscs and cm significantly reduced fibrosis and significantly increased bcl-2 expression levels in the myocardium compared to the dox group (p<0.01). however, there was no significant difference between bax expression levels in these groups. in addition, secretion of hgf and igf-1 was detected in the cm (p<0.01). conclusion: we conclude that intravenous transplantation of mscs and cm can attenuate myocardial fibrosis and increase bcl-2 expression. this may be mediated by paracrine signaling from mscs via anti-fibrotic and anti-apoptotic factors such as hgf and igf-1.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis

OBJECTIVE The aim of this study was to test the effect of intravenous injection of mesenchymal stem cells (MSCs) on doxorubicin (DOX)-induced fibrosis in the heart. We investigated the mechanisms that possibly mediate this effect. MATERIALS AND METHODS In this experimental study, fibrosis in the myocardium of adult male Wistar rats (weights 180-200 g, 9-10 weeks of age, total n=30) was create...

full text

Regenerative Effect of Bone Marrow-derived Mesenchymal Stem Cells in Thioacetamide-induced Liver Fibrosis of Rats

The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of th...

full text

Evaluation of age effects on doxorubicin-induced toxicity in mesenchymal stem cells

Background: Doxorubicin, by aggregating in bone marrow, causes genotoxic effects, and thus reduces the repair ability of cells. The present study was conducted as an in vitro evaluation of age effects on the cytotoxicity induced by doxorubicin in mesenchymal stem cells (MSCs). &nbsp;&nbsp; Methods: The MSCs of female BALB/c mice aged 1, 8, and 16 months were separated, characterized, and subse...

full text

Therapeutic Potential of Mouse Bone Marrow Mesenchymal Stem Cells in Carbon Tetrachloride (Ccl4)-Induced Liver Fibrosis

Purpose: To study the effect of allogenic bone marrow mesenchymal stem cells (BMMSCs) transplantation on carbon tetrachloride-induced liver fibrosis in mice. Materials and Methods: Fifty five female NMRI mice were divided in 5 groups, and to induce liver fibrosis CCL4 intraperitonealy was injected 1ml/Kg twice a week for 8 weeks 106 allogenic BMMSCs were infused in cell therapy group via tail v...

full text

Effect of Antioxidants and Mesenchymal Stem Cells on Cisplatin Induced Renal Fibrosis in Rats

Inflammation of the tubulointerstitial compartment, leading to fibrosis, is a major factor in the progressive loss of renal function. About 80% of total kidney volume is composed of tubular epithelial cells and cells within the interstitial space. There are also a small number of resident mononuclear cells and fibroblasts. It is widely recognized that progressive renal disease is accompanied by...

full text

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

My Resources

Save resource for easier access later


Journal title:
cell journal

جلد ۱۴، شماره ۲، صفحات ۱۴۲-۱۵۱

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023