tissue uptakes of 67ga-bleomycin and carrier free 67ga in fibrosarcoma-bearing mice

Authors

faraj tabeie

b bolouri

amir reza jalilian

n mosaffa

hossein rajabi

abstract

67gallium-bleomycin complex (67ga-blm) was prepared using thakour method. radio-thin-layer-chromatography of prepared complex showed a2 and b2 radiopeaks with rf at 0.7 and 0.4 respectively with a purity of above % 95. tissue uptake of 67ga-blm and 67gacl3 in twelve tissues including tumor, blood, liver, lung, spleen, muscle, skin, heart, kidney, colon, colon content ,bladder and the total body were counted by well counter at 1, 2, 4, 24 and 48 hours post injection of radiopharmaceuticals. uptakes of tissues are expressed as percent injected dose per gram of tissue. the clearance rate of 67ga-blm was 1.75-1.95 times faster than 67gacl3 at all time intervals. bladder uptakes of 67ga-blm were highest among twelve tissues at 1,2 and 4 hours after injection, then falling rapidly after 24 and 48 hours. blood uptake of 67ga-blm was lower than 67gacl3 in all time intervals. colon content uptake of 67ga-blm was highest among twelve tissues at 2 and 4 hours post injection. tumor to tissue activity ratios were also calculated, showing an increase of tumor to blood and muscle ratios. tumor to blood ratio increased from 0.3 at 1 hour to 5.3 at 48 hours. activity ratio of muscle increased from 0.5 at 1 hour to 5.5 at 48 hours. whole body counting of animals showed that effective half lives of 67ga-blm and 67gacl3 were about 1 and 15 hours respectively, which renders faster excretion of 67ga-blm complex. biodistribution data clearly indicates that prepared complex in comparison with carrier free 67ga (67gacl3) has two main advantages: 1) high tumor to soft tissue uptake ratio that make it suitable for tumor imaging, 2) faster excretion specially at first three hours post injection. in addition complex is stable in vitro and in vivo.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Tissue uptakes of 67Ga-bleomycin and carrier free 67Ga in fibrosarcoma-bearing mice

67Gallium-bleomycin complex (67Ga-BLM) was prepared using Thakour method. Radio-thin-layer-chromatography of prepared complex showed A2 and B2 radiopeaks with Rf at 0.7 and 0.4 respectively with a purity of above % 95. Tissue uptake of 67Ga-BLM and 67GaCl3 in twelve tissues including tumor, blood, liver, lung, spleen, muscle, skin, heart, kidney, colon, colon content ,bladder and the tota...

full text

Dynamic Distribution of 67Ga-Bleomycin Complex and Carrier Free 67Gallium in Normal Mice

This study reports the labeling of Gallium-Bleomycin (67Ga-BLM) complex as a radiopharmaceutical and optimization of its labeling conditions; pH, reaction time, temperature, concentration of bleomycin and its biodistribution in normal Bulb C mice. The biodistribution of the complex was compared with 67Ga-Cl3 in 11 selected organs including blood, liver, lung, spleen, muscle, skin, heart, kidney...

full text

57Co-bleomycin and 67Ga-citrate in detecting and staging lung cancer.

In the investigation of suspected lung cancer bleomycin labelled with cobalt-57 and gallium-67 labelled with citrate are currently used to detect the primary tumour and to establish the presence of metastases in the lung hilum and mediastinum. A comparative study of these radio-pharmaceuticals was performed in 63 patients with proved lung cancer. 57Co-bleomycin showed the primary tumour in 58 p...

full text

Production and evaluation of [67Ga]-DTPA-Rituximab

Background: In order to obtain an anti-CD20 conjugate to be used in future therapeutic studies with therapeutic radioisotopes, 67Ga-labeled antibody was prepared as a model of metal chelated immunoconjugate for preliminary dosimetric and biodistribution studies. Materials and Methods: Rituximab was labeled with [67Ga]-gallium chloride after residulation with freshly prepared cyclic DTP...

full text

Effect of gallium nitrate on the in vivo distribution of 67Ga in rats bearing inflammatory lesions.

Effect of gallium nitrate on the pharmacokinetics and tissue accumulation of 67Ga was investigated in rats bearing turpentine oil-induced abscess. Gallium nitrate accelerated the blood disappearance of 67Ga, but this effect was less potent than that of ferric nitrate. The accumulation of 67Ga in liver was significantly lowered by gallium nitrate, but no significant decrease of 67Ga accumulation...

full text

Production and Quality Control of [67Ga]-DOTA-trastuzumab for Radioimmunoscintigraphy

Breast cancer radioimmunoscintigraphy targeting HER2/neu expression is a growing field of work in nuclear medicine research. In this study, trastuzumab was successively labeled with [(67)Ga] GaCl3 after conjugation with DOTA-NHS-ester. The conjugates were purified by molecular filtration, the average number of DOTA conjugated per mAb was calculated and total concentration was determined by spec...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of nuclear medicine

Publisher: tehran university of medical sciences

ISSN 1681-2824

volume 12

issue 2 2004

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023