improvement of vitrification of in vitro produced buffalo embryos with special reference to sex ratio following vitrification
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abstract
cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. to improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. the first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. morphologically normal embryos and survival rates (re-expansion) significantly increased when vitrified morulae were exposed for 2 min compared to 3 min (p
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Improvement of vitrification of in vitro produced buffalo embryos with special reference to sex ratio following vitrification
Cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. To improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. The first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. Morphol...
full textImprovement of vitrification of in vitro produced buffalo embryos with special reference to sex ratio following vitrification.
Cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. To improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. The first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. Morphol...
full textPost-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification
The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium containing cytochalasin-b (cyto-b) in a CO(2) incubator for 40 min for microfilament stabilization and were cryopreserved by a two-step vitrification method at 24 degrees C ...
full textLipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification.
UNLABELLED The objective was to evaluate supplementation of fetal calf serum (FCS) and phenazine ethosulfate (PES), a metabolic regulator that inhibits fatty acid synthesis, in culture media during in vitro production (IVP) of bovine embryos. Taking oocyte fertilization (n = 4,320) as Day 0, four concentrations of FCS (0, 2.5, 5, and 10%) and three periods of exposure to PES (without addition-C...
full textTiming of The First Zygotic Cleavage Affects Post-Vitrification Viability of Murine Embryos Produced In Vivo
Background Timing of the first zygotic cleavage is an accurate predictor of embryo quality. Embryos that cleaved early (EC) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (LC). However, the viability of EC embryos in comparison to LC embryos after vitrification is unknown. The present study aims to investigate the post-vitrification de...
full textEfficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
BACKGROUND Currently, the cryopreservation of embryos and oocytes is essential for assisted reproductive technology (ART) laboratories worldwide. This study aimed to evaluate the efficacy of the Kitasato Vitrification System (KVS) as a vitrification device for the cryopreservation of mouse embryos to determine whether this novel device can be adapted to the field of ART. METHODS In Experiment...
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Journal title:
iranian journal of veterinary researchPublisher: shiraz university
ISSN 1728-1997
volume 16
issue 4 2015
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