a single radial haemolysis technique for rapid diagnosis of goat pox diseases

A Single Radial Haemolysis Technique for Rapid Diagnosis of Goat Pox Diseases

Sheep Pox and Goat Pox

Sheep pox and goat pox are viral diseases of sheep and goats characterised by fever, generalised papules or nodules, vesicles (rarely), internal lesions (particularly in the lungs), and death. Both diseases are caused by strains of capripoxvirus, all of which can infect sheep and goats. Although most of the strains examined cause more severe clinical disease in either sheep or goats, some strai...

Evaluation of the single radial haemolysis (SRH) technique for rubella antibody measurement.

Sera from 1258 individuals have been tested by four laboratories for rubella antibody by both the haemagglutination-inhibition and single radial haemolysis techniques. There was good agreement between the results obtained by the two methods. Although sheep red blood cells were used in the single radial haemolysis plates, no problems were encountered with sera from patients with infectious monon...

The use of single-radial-haemolysis for rubella antibody studies.

The use of a single-radial-haemolysis technique for the detection of antibody to rubella virus is described. The single-radial-haemolysis test was compared with the standard HI methods for the detection of antibody to rubella virus. A close correlation between the two methods was observed in a survey of over two thousand serum samples and the study indicated that single-radial-haemolysis was hi...

Production of Goat Pox Virus Vaccine from a Live Attenuated Goat Pox Virus Strain

Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days. Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers. Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation. TCID50 calculated for the pool virus was 10 / ml. Vaccine was found free from any bacterial ...

LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples

Animal species detection is one of the crucial steps for consumer’s food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Gree...