restriction enzyme analysis of thymidine kinase gene in four iranian isolates of herpes simplex virus type 1

Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1

PCR detection of thymidine kinase gen of latent herpes simplex Virus type 1 in mice trigeminal ganglia

  Herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. During latent infection, virus genome exhibit limited transcription, with the HSV LATs consistently detected in latency infected ganaglia. Following ocular infection viral latency develops in the trigeminal ganglia. In this study PCR has been used for detection of HSV-1 nuc...

Kinetic studies of herpes simplex virus type 1-encoded thymidine and thymidylate kinase, a multifunctional enzyme.

Kinetic studies of the reactions catalyzed by herpes simplex virus type l-encoded thymidine and thymidylate multifunctional kinase indicate that the addition of substrates (thymidine and ATP*Mg’+, or thymidylate and ATP l Mg2+) to the enzyme is random, while release of the products (thymidylate and ADP*M8+, or thymidine diphosphate and ADP*Mg+) is ordered, with ADP.Mg2+ being released first. Th...

pcr detection of thymidine kinase gen of latent herpes simplex virus type 1 in mice trigeminal ganglia

herpes simplex virus type 1 establishes a latent infection in the peripheral nervous system following primary infection. during latent infection, virus genome exhibit limited transcription, with the hsv lats consistently detected in latency infected ganaglia. following ocular infection viral latency develops in the trigeminal ganglia. in this study pcr has been used for detection of hsv-1 nucli...

A Preliminary Study on the Molecular Identification of Herpes Simplex Virus Type 1 in Iranian Population

Herpes Simplex Virus Type 1 (HSV-1) is a member of the Herpesviridae family that causes herpetic disease in human. During the last 25-30 years, many investigations have been conducted on pathogenesis and molecular biology of this virus in several countries. In Iran HSV-1 has been isolated from patients and detected using immunological techniques. In this study, we investigated the molecular asp...

Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy.

Herpes simplex virus type 1 (HSV-1) thymidine kinase is currently used as a suicide agent in the gene therapy of cancer. This therapy is based on the preferential phosphorylation of nucleoside analogs by tumor cells expressing HSV-1 thymidine kinase. However, the use of HSV-1 thymidine kinase is limited in part by the toxicity of the nucleoside analogs. We have used random sequence mutagenesis ...