chemical composition and biological activities of methanolic extract of scrophularia oxysepala boiss

isolated five known iridoids glycosides (scrophuloside a, harpagoside b, 5-hydroxyloganin, 8-o-acetylharpagide and 6-o-methyl,1-glucopyranosyl catalpol ),one phenyl ethanoid glycoside (verbascoside) and a phenyl ethanol amine (2-(4-chlorobenzyl amino) ethanol ) compound from the methanolic extract of aerial parts of scrophularia oxysepala using by high performance liquid chromatography based on isocratic and liner gradients by c18 column . the structure elucidations of the isolated compounds were performed by spectroscopic methods include 1h-nmr, 13c-nmr, 2 d nmr technique such as hmbc ( in deuterated methanol as solvent) , gc-ms and uv also methanolic extract and fractions ( fractionated on solid phase extraction on c18 cartridge(spack-c18)) of this plant was tested for free radical scavenging properties toward the 1, 1-diphenyl-1-picrylhydrazyl (dpph), general toxicity (brine shrimp toxicity assay) , insecticidal ( contact toxicity insecticidal assay) and antimalarial activities (hemebiocrystallization inhibition assay).