protective effect of 25mg-porphyrin-fullerene nanoparticles on oxygen-glucose deprivation/reperfusion injury in pc12 cells

we investigated the effects of 25 mg-porphyrin-fullerene nanoparticles, ( 25 mgpmc16) smart ferroporphyrin nanoparticles, on pc12 cells exposed to oxygen-glucose deprivation/reperfusion. in order to explore its effect on cells under oxygen-glucose deprivation conditions, the cultures were pretreated with 25 mgpmc16 24 hours prior to oxygen-glucose deprivation/reperfusion. to initiate the oxygen-glucose deprivation/reperfusion, the cell culture medium was replaced with a glucose-free medium and the cells were transferred to a humidified incubation chamber in a mixture of 95% n 2 and 5% co 2 at 37° c for 30, 60 and 120 min. cell viability was assessed by mtt assay. exposure of pc12 cells to 30, 60 and 120 min oxygen-glucose deprivation significantly decreased the cell viability. pretreatment of the cultures with 25 mgpmc16 significantly increased cell viability in a concentration-dependent manner. pretreatment, the cultures with mk-801 (10 µm), a non-competitive nmda antagonist, has attenuated the cell death after 30 min oxygen-glucose deprivation. we concluded that 25 mgpmc16 could protect pc12 cells against oxygen-glucose deprivation/reperfusion-induced cell injury in a concentration-dependent manner. that could be due to the effect of 25 mgpmc16 on atp synthesis and the antioxidant effects of its components.