high performance liquid chromatography coupled with pre-column derivatization for determination of oxidized glutathione level in rats exposed to paraquat
Authors
abstract
glutathione (gsh) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ros) which oxidizes to glutathione disulfide (gssg). paraquat (pq), awidely used herbicide, causes pulmonary injury with the productionof ros. excessive ros accumulation as a consequence of pq exposure are frequently targeted by gsh thereby oxidative stress leads to depletion of cellular gsh by transforming of gsh to glutathione disulfide (gssg). a precise method of measuring of gssg concentration in plasma as indicator of oxidative stress is needed. some analytical techniques such as high-performance liquid chromatography (hplc), gas chromatography and capillary electrophoresis have been used for determination of gssg concentration. in the present study, a new hplc method with fluorescence detection based on derivatization of the amine group of glutathione with 9-fluorenylmethyl chloroformate (fmoc-cl) was developed. male wistar albino rats exposed to different doses of pq (20-60 mg/kg) and control group were used and after protein precipitation, their plasma was subjected to derivatization with fmoc in the presence of borate buffer. the derivatized samples were injected to hplc system with c18 column, mobile phase consisting of methanol and phosphate buffer, λem= 315 nm, λex = 260 nm. among all experimental groups, the rats which received 60 mg/kg pq, showed a significant increase in the amount of oxidized glutathione (gssg) compared to the control group. in this study, the applied derivatization and hplc method made it possible to measure small amounts of glutathione in plasma using a precise and sensitive technique.
similar resources
High Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat
Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...
full textHigh Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat
Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...
full textHigh Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat
Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...
full textDetermination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection
Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...
full textAutomated pre-column derivatization and its application to amino-acid analysis using high-performance liquid chromatography
Although chemical modification of analytes is a well-accepted adjunct to chromatography, little attention had been paid to the automation of pre-column derivatization techniques. An automated pre-column system would offer advantages when analyses are performed using labile derivatives; and also when the time between injections is long and so staff time spent on achieving maximum analytical outp...
full text[Development of highly sensitive determination of biogenic compounds by high-performance liquid chromatography with pre-column fluorescence derivatization].
A sensitive fluorescent labeling reagent, 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride (DMS-Cl), for the determination of amino compounds in HPLC was developed. DMS-Cl reacted with amino compounds in the basic medium to produce the corresponding fluorescent sulfonamides (excition 318 nm, emission 406 nm in aqueous acetonitrile). When amino acids were analyzed using revers...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of pharmaceutical researchجلد ۱۲، شماره ۴، صفحات ۹۱۱-۹۱۶
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023