enhanced production of insulin-like growth factor i protein in escherichia coli by optimization of five key factors
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abstract
abstract human insulin-like growth factor i (higf-i) is a kind of growth factor with clinical significance in medicine. the major objective of this study is over- production of recombinant human insulin-like growth factor i( rhigf-i) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. up to now e. coli expression system has been widely used as a host to produce rhigf-1 with high yields. batch cultures as non-continuous fermentation were carried out to overproduce rhigf-i in e. coli. the effects of culture medium type, induction temperature and amount of inducer on cell growth and igf-i production were investigated in shaking flask. taguchi design of experiments (doe) method was used as the statistical method. analysis of experimental data showed that maximum production of rhigf-i was occurred in 32y culture medium at 28°c and 0.05 mm iptg. under this condition, 0.7 g/l of rhigf-i was produced as the inclusion bodies. following optimization of these three factors, we have also optimized the amount of glucose and induction time in 5 liter top bench bioreactor. full factorial design of experiment method was used for these two factors as the statistical method. 10 g/l and od600=5 were selected as the optimum point of glucose amount and induction time, respectively. finally we have had 1.26 g/l rhigf-1 production as the final product that is one of the best reported amounts.
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Journal title:
iranian journal of pharmaceutical researchجلد ۱۴، شماره ۳، صفحات ۹۰۷-۹۱۷
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