molecular characterization of fasciola hepatica isolates by rapd-pcr and ribosomal its1 sequencing
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Molecular differentiation of sheep and cattle isolates of Fasciola hepatica using RAPD-PCR
Understanding genetic structure and status of genetic variation of Fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. Random amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variation of F. hepatica in sheep and cattle. DNA was extracted from adult helminthes removed from livers of each infected ani...
full textmolecular differentiation of sheep and cattle isolates of fasciola hepatica using rapd-pcr
understanding genetic structure and status of genetic variation of fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. random amplified polymorphic dna (rapd-pcr) was used to study the genetic variation of f. hepatica in sheep and cattle. dna was extracted from adult helminthes removed from livers of each infected ani...
full textGenetic Characterization of Fasciola Isolates from West Azerbaijan Province Iran Based on ITS1 and ITS2 Sequence of Ribosomal DNA.
BACKGROUND Fascioliasis, caused by Fasciola hepatica and F. gigantica, has medical and economic importance in the world. Molecular approaches comparing traditional methods using for identification and characterization of Fasciola spp. are precise and reliable. The aims of current study were molecular characterization of Fasciola spp. in West Azerbaijan Province, Iran and then comparative analys...
full textMolecular Identification and Differentiation of Fasciola Isolates Using PCR- RFLP Method Based on Internal Transcribed Spacer (ITS1, 5.8S rDNA, ITS2)
BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...
full textgenetic characterization of fasciola isolates from west azerbaijan province iran based on its1 and its2 sequence of ribosomal dna
abstract background: fascioliasis, caused by fasciola hepatica and f. gigantica , has medical and economic importance in the world. molecular approaches comparing traditional methods using for identification and characterization of fasciola spp. are precise and reliable. the aims of current study were molecular characterization of fasciola spp. in west azerbaijan province, iran and then compar...
full textMolecular characterization of Pasteurella multocida isolates obtained from poultry, ruminant, cats and dogs using RAPD and REP-PCR analysis
In the present study, Randomly Amplified Polymorphic DNA (RAPD) and Repetitive Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP-PCR) were used to characterize 131 isolates of Pasteurella multocida, originating from different healthy and diseased animal species obtained from several geographical regions of Iran. The RAPD and REP-PCR generated amplified products in the range ...
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Journal title:
iranian red crescent medical journalجلد ۲۰۱۶، شماره ۱۱، صفحات ۲۷-۳۲
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