Diagnostic RAS mutation analysis by polymerase chain reaction (PCR)
نویسنده
چکیده
RAS mutation analysis is an important companion diagnostic test. Treatment of colorectal cancer with anti-Epidermal Growth Factor Receptor (EGFR) therapy requires demonstration of RAS mutation status (both KRAS and NRAS), and it is good practice to include BRAF. In Non-Small Cell Lung Cancer (NSCLC) and melanoma, assessment of RAS mutation status can be helpful in triaging patient samples for more extensive testing. This mini-review will discuss the role of PCR methods in providing rapid diagnostic information for cancer patients.
منابع مشابه
Diagnosis of pancreatic lesions using fine needle aspiration cytology: detection of K-ras point mutations using solid phase minisequencing.
AIMS To improve the diagnostic value of fine needle aspiration biopsy of pancreatic lesions using a simple mutation detection method based on the polymerase chain reaction (PCR). METHODS Fine needle aspirates from 21 suspected pancreatic lesions were analysed for K-ras codon 12 point mutations using solid phase minisequencing. RESULTS A point mutation in codon 12 of the K-ras gene was detec...
متن کاملBRAFV600E mutation testing in fine needle aspirates of thyroid nodules: potential value of real-time PCR.
The BRAF(V600E) mutation is a valuable adjunctive diagnostic tool to ultrasound (US)-guided fine-needle aspiration (US-FNA). The objective of this study was to investigate the potential value of realtime PCR to detect BRAF(V600E) mutation. This study included 447 thyroid nodules in 420 patients who underwent US-FNA and BRAF(V600E) mutation analysis using dual priming oligonucleotide-based multi...
متن کاملPolymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum
Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes, and also the smallest organisms that can live independently. They are able to cause serious and chronic disease because of some unique characteristics. Mycoplasma gallisepticum (MG) is an important avian pathogen causing significant economical losses within the poultry industry. The...
متن کاملPolymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT
Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 ...
متن کاملK-ras mutations in the plasma correspond to computed tomographic findings in patients with pancreatic cancer.
To the Editor: The prognosis of pancreatic carcinoma has not improved in the past 20 years. Despite the recent advances in diagnostic techniques, it remains difficult to diagnose this devastating disease in the early stage. Developing informative noninvasive cancer biomarkers is essential to achieve early detection of tumors to better stratify patients’ prognosis and to optimize therapy for ind...
متن کامل