Plasma-based Surface Functionalization of Polystyrene Substrate
نویسندگان
چکیده
The method to modify the surface of polystyrene (PS) substrate for stable and versatile biomolecule immobilization was developed, which consists of two steps: 1) surface treatment by argon plasma irradiation to introduce hydroxyl group on PS and 2) covalent immobilization of vinylmethyleter-maleic acid copolymer (VEMAC) through a coupling reaction between hydroxyl group on plasma-irradiated PS and carboxyl group of VEMAC. For cell culture application, the cell adhesive GRGDS peptide was conjugated with VEMAC immobilized on PS substrate. The cell culture experiments using NIH3T3 (mouse embryonic fibroblast) indicated that the GRGDS conjugated with VEMAC was specifically recognized by cell-surface integrin and promoted the adhesion and proliferation.
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