Community structure analysis of methanogens associated with rumen protozoa reveals bias in universal archaeal primers.
نویسندگان
چکیده
The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification.
منابع مشابه
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 78 11 شماره
صفحات -
تاریخ انتشار 2012