Expression, purification, and characterization of the recombinant putative periplasmic hemin-binding protein (HutB) of Photobacterium damselae subsp. piscicida.

نویسندگان

  • Francesca Andreoni
  • Romina Boiani
  • Giordano Serafini
  • Irene Bianconi
  • Sabrina Dominici
  • Francesca Gorini
  • Mauro Magnani
چکیده

HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affinity (K(d)) of 10 microM.

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عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 73 5  شماره 

صفحات  -

تاریخ انتشار 2009