A Thermodynamic Study on Hydration and Dehydration of DNA and RNA −Amphiphile Complexes

In the current opinion, we would like to study adhesion of DNA and RNA−amphiphile complexes to non–modified glasses, glasses containing phenylboronic acid (PBA) attached via 3– glycidoxypropyltrimethoxysilane (GPTMS) [(PBA–GPTMS–glasses)] and glasses containing grafted dimethyllacrylamide–acrylamide phenylboronic acid (DMAA–AAPBA) copolymer (DMAA–AAPBA– glasses). We prepared glasses modified with GPTMS and then attached PBA to them. After that, we modified glasses by (3–mercaptoropyl) trimethoxy silane (MPTMS) and grafted DMAA–AAPBA copolymer to the modified glass plates. For increasing the measuring sensitivity, DNA and RNA−amphiphile complexes were stained with Procion Red HE3B, Red 4GE, Red 510, Red KE3B, Red 120, Red ABO, Red A4G, Red 3B, Red KE3B, Red HE3G, Red 53 L, Red HE3BI, Red E3B, Red CT3B, Red ES3B, Red LHE3B, Red I3B, Red HE3BFC, Red MEB, Red SE3BI, Red 24990, Red SE3B, Red 3BHE and other active and reactive dyes. DNA and RNA−amphiphile complexes stained with Procion Red HE3B, Red 4GE, Red 510, Red KE3B, Red 120, Red ABO, Red A4G, Red 3B, Red KE3B, Red HE3G, Red 53 L, Red HE3BI, Red E3B, Red CT3B, Red ES3B, Red LHE3B, Red I3B, Red HE3BFC, Red MEB, Red SE3BI, Red 24990, Red SE3B, Red 3BHE and other active and reactive dyes formed denser and more stable layer on the copolymer–grafted supports compared onto supports modified with phenylboric acid via a organosilane spacer [1–18]. Adhesion of DNA and RNA−amphiphile complexes to boronate–containing polymer fixed on solid support in different conditions such as pH, precipitation time and different concentrations of glucose (dextrose), fructose (levulose) and galactose were studied.