Investigations of excitability of isolated and non-isolated neurons from the terminal ganglion of Periplaneta americana by current/voltage clamp and intracellular perfusion.
نویسندگان
چکیده
It was shown by means of current and voltage clamp measurements in combination with the intracellular perfusion method that the isolated motoneuron nerve cell bodies of the cockroach Periplaneta americana show overshoot action potentials. A regenerative Na current was found to be responsible for the excitability of these membranes; however, the results are not in agreement with a de novo synthesis of these channels.
منابع مشابه
Modulation of Low-Voltage-Activated Inward Current Permeable to Sodium and Calcium by DARPP-32 Drives Spontaneous Firing of Insect Octopaminergic Neurosecretory Cells
Identification of the different intracellular pathways that control phosphorylation/dephosphorylation process of ionic channels represents an exciting alternative approach for studying the ionic mechanisms underlying neuronal pacemaker activity. In the central nervous system of the cockroach Periplaneta americana, octopaminergic neurons, called dorsal unpaired median (DUM; DUM neurons), generat...
متن کاملA New ZVZCS Isolated Dual Series Resonant DC-DC Converter with EMC Considerations
A novel ZVZCS isolated dual series-resonant active-clamp dc–dc converter is proposed to obtain high efficiency. The proposed converter employs an active-clamp technique, while a series-resonant scheme controls the output voltage with the complementary pulse width modulation controller. The active-clamp circuit serves to recycle the energy stored in the leakage inductance or the magnetizing ...
متن کاملNervous System of Periplaneta americana Cockroach as a Model in Toxinological Studies: A Short Historical and Actual View
Nervous system of Periplaneta americana cockroach is used in a wide range of pharmacological studies, including electrophysiological techniques. This paper presents its role as a preparation in the development of toxinological studies in the following electrophysiological methods: double-oil-gap technique on isolated giant axon, patch-clamp on DUM (dorsal unpaired median) neurons, microelectrod...
متن کاملElectrophysiological study of amygdale-induced changes in the excitability of CA1 hippocampal pyramidal neurons in male adult rats
Introduction: Many studies have shown that amygdala kindling produces synaptic potentiation by induction of changes in the neuronal electrophysiological properties and inward currents both in epileptic focus and in the areas which are in connection with the epileptic focus and have important role in seizure development and progression such as hippocampal CA1 region. However, cellular mechani...
متن کاملPharmacological properties of axonal sodium channels in the cockroach Periplaneta americana L. I. Selective block by synthetic saxitoxin.
Voltage-clamp experiments on isolated giant axons of the cockroach Periplaneta americana L. show that chemically synthesized saxitoxin specifically and reversibly blocks the transient inward sodium current without affecting the steady-state outward potassium current. From the concentration depending of sodium current suppression it is concluded that individual sodium channels are blocked by sin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- General physiology and biophysics
دوره 8 6 شماره
صفحات -
تاریخ انتشار 1989