Enzymatic Decarboxylation of Oxalic Acid* by William
نویسندگان
چکیده
Although it has been recognized that oxalic acid is decomposed by a variety of microorganisms (2-6), relatively little is known of the details of oxalic acid metabolism. The present study represents an attempt to establish the mechanism of oxalate degradation at the enzyme level by the use of a bacterium isolated from soil. Partially purified enzyme preparations obtained from the organism catalyzed the decarboxylation of oxalic acid with the formation of formic acid and carbon dioxide. The system requires substrate quantities of ATP’ and catalytic amounts of acetate, CoA, ThPP, and magnesium ions. Acetate, CoA, ATP, and magnesium ions are required for the formation of acetyl CoA (Equation 1) which is in turn utilized according to the over-all reaction described by Equation 2.
منابع مشابه
Enzymatic decarboxylation of oxalic acid.
Large quantities of oxalic acid accumulate in the cell sap of various plants and as a major product of carbohydrate metabolism by many molds. A recent investigation in our laboratory revealed that oxalic acid was produced from oxalacetic acid by an enzyme system which was isolated from the mycelia of Aspergillus niger (1). It was also demonstrated that oxalic acid was converted to formic acid a...
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