Improved thermostability of Clostridium thermocellum 1 endoglucanase Cel 8 A using consensus - guided mutagenesis 2 3
نویسندگان
چکیده
3 Michael Anbar, Ozgur Gul, Raphael Lamed, Ugur O. Sezerman 4 and Edward A. Bayer* 5 6 Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 7 76100 Israel 8 2 Biological Sciences and Bioengineering, Sabanci University, Orhanli, Tuzla, 9 Istanbul, Turkey 10 3 Department of Molecular Microbiology and Biotechnology, Tel Aviv University, 11 Ramat Aviv 69978 Israel; 12 13 Running Title: Improved thermostability of endoglucanase Cel8A 14 15 *Corresponding author 16 Edward A. Bayer 17 Tel: (+972)-8-934-2373 18 Fax: (+972)-8-946-8256 19 Email: [email protected] 20 21 22
منابع مشابه
Enhancement of cellulosome-mediated deconstruction of cellulose by improving enzyme thermostability
BACKGROUND The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type enzymes. Exoglucanase Cel48S and endoglucanase Cel8A, both key elements of the natural cellulosome from this bacterium, were engineered previously for increased thermostabil...
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متن کاملEngineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase.
The Clostridium thermocellum cellobiose phosphorylase (CtCBP) is a large protein consisting of 812 amino acids and has great potential in the production of sugar phosphates, novel glycosides, and biofuels. It is relatively stable at 50 °C, but is rapidly inactivated at 70 °C. To stabilize CtCBP at elevated temperatures, two protein-engineering approaches were applied, i.e. site-directed mutagen...
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The growing demands of bioenergy has led to the emphasis on novel cellulases to improve efficiency of biodegradation process of plant biomass. Therefore, a thermostable cellulolytic gene (CenC) with 3675 bp was cloned from Clostridium thermocellum and over-expressed in Escherichia coli strain BL21 CodonPlus. It was attested that CenC belongs to glycoside hydrolase family 9 (GH9) with four bindi...
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