Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006-2007.
نویسندگان
چکیده
In the last 5 years, there has been only one reported human case of West Nile virus (WNV) disease in northern Mexico. To determine if the virus was still circulating in this region, equine and entomological surveillance for WNV was conducted in the state of Nuevo Leon in northern Mexico in 2006 and 2007. A total of 203 horses were serologically assayed for antibodies to WNV using an epitope-blocking enzyme-linked immunosorbent assay (bELISA). Seroprevalences for WNV in horses sampled in 2006 and 2007 were 26% and 45%, respectively. Mosquito collections in 2007 produced 7365 specimens representing 15 species. Culex mosquitoes were screened for WNV RNA and other genera (Mansonia, Anopheles, Aedes, Psorophora and Uranotaenia) were screened for flaviviruses using reverse-transcription (RT)-PCR. Two pools consisting of Culex spp. mosquitoes contained WNV RNA. Molecular species identification revealed that neither pool included Culex quinquefasciatus (Say) (Diptera:Culicidae) complex mosquitoes. No evidence of flaviviruses was found in the other mosquito genera examined. These data provide evidence that WNV is currently circulating in northern Mexico and that non-Cx. quinquefasciatus spp. mosquitoes may be participating in the WNV transmission cycle in this region.
منابع مشابه
Detection of antibodies to West Nile and Saint Louis encephalitis viruses in horses.
To the editor: West Nile virus (WNV) and Saint Louis encephalitis virus (SLEV) belong to the Japanese encephalitis an-tigenic complex (family Flaviviridae, ge-nus Flavivirus). 1-3 As with other members of this complex, WNV and SLEV are usually maintained in cycles between birds and Culex species mosquitoes. Humans, horses and other mammals are typically dead-end hosts. WNV and SLEV infections i...
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ورودعنوان ژورنال:
- Medical and veterinary entomology
دوره 26 3 شماره
صفحات -
تاریخ انتشار 2012