Porous/Gold surface Preparation for Protein Microarray Applications
نویسندگان
چکیده
Surface chemistry is extremely important in microarray technology, since it determines the successful attachment of the molecules, spot morphology or signal intensity. This paper describes development of functionalised gold layer surfaces deposited onto porous silicon as substrates for microarray experiments. Two gold textures were studied in order to find the best ratio between signal intensity and spot morphology. The experiments revealed: (i) that porous silicon substrate ensures spots uniformity and good response of the signal intensity with respect to concentration; (ii) saturation bands for high concentrations for both types of substrates. Key-words: protein microarray, porous silicon, gold layer.
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