CREB, ATF, and AP-1 transcription factors regulate IFN-gamma secretion by human T cells in response to mycobacterial antigen.

CREB, ATF, and AP-1 Transcription Factors Regulate IFN- Secretion by Human T Cells in Response to Mycobacterial Antigen

Phosphodiesterase 4 inhibitors prevent cytokine secretion by T lymphocytes by inhibiting nuclear factor-kappaB and nuclear factor of activated T cells activation.

Blockade of phosphodiesterase 4 with rolipram reduced the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-5, IL-10, and IL-2 but poorly inhibited cell proliferation and interferon-gamma (IFN-gamma) production by activated human T cells. Addition of dibutyryl cAMP mimicked rolipram inhibitions on proliferation, IL-2, TNF-alpha, and IFN-gamma but not on IL-10 or IL-5 production....

Serum responsiveness of the rat PCNA promoter involves the proximal ATF and AP-1 sites.

We have previously shown that the rat PCNA (proliferating cell nuclear antigen) gene promoter is responsive to serum stimulation. In this study, the sequence of the promoter responsive to serum stimulation has been localized in the region between nucleotides -70 and +125 relative to the transcription initiation site. This region contains an ATF site (nucleotides -51 to -44) and an AP-1 site (nu...

Cyclic AMP response element-binding protein positively regulates production of IFN-gamma by T cells in response to a microbial pathogen.

IFN-gamma is essential for resistance to many intracellular pathogens, including Mycobacterium tuberculosis. Transcription of the IFN-gamma gene in activated T cells is controlled by the proximal promoter element (-73 to -48 bp). CREB binds to the IFN-gamma proximal promoter, and binding is enhanced by phosphorylation of CREB. Studies in human T cell lines and in transgenic mice have yielded co...

ارزیابی پرولیفراسیون سلولی و بیان داخل سلولی اینترفرون گاما (IFN-Gamma) در بیماران مبتلا به بروسلوز

T cell proliferation is a standard method to evaluate cellular immune responses against intracellular infectious agents. Recently, intracellular cytokine assay is a valuable procedure for studying of the immune response to various stimuli such as intracellular microbes. The present study was undertaken to assess cell-mediated    immune responses in patients with acute ...