Multiple reaction monitoring to identify site-specific troponin I phosphorylated residues in the failing human heart.

Multiple Reaction Monitoring to Identify Site-Specific Troponin I Phosphorylated Residues in the Failing Human Heart Running title: Zhang et al., Targeted quantitation of cardiac troponin I phosphorylation

Heart Failure Multiple Reaction Monitoring to Identify Site-Specific Troponin I Phosphorylated Residues in the Failing Human Heart

Background—Human cardiac troponin I is known to be phosphorylated at multiple amino acid residues by several kinases. Advances in mass spectrometry allow sensitive detection of known and novel phosphorylation sites and measurement of the level of phosphorylation simultaneously at each site in myocardial samples. Methods and Results—On the basis of in silico prediction and liquid chromatography/...

Targeted proteomics for determining phosphorylation site-specific associations in cardiovascular disease.

Posttranslational modifications (PTMs) are chemical or physical changes to proteins that can alter structure/ function relationships and thus influence catalysis, cell localization, and interactions with other proteins and biomolecules. There are 200 recognized PTMs, generating a vast scope for altering protein function and increasing the potential for complex regulatory crosstalk between diver...

PKCα-Specific Phosphorylation of the Troponin Complex in Human Myocardium: A Functional and Proteomics Analysis

AIMS Protein kinase Cα (PKCα) is one of the predominant PKC isoforms that phosphorylate cardiac troponin. PKCα is implicated in heart failure and serves as a potential therapeutic target, however, the exact consequences for contractile function in human myocardium are unclear. This study aimed to investigate the effects of PKCα phosphorylation of cardiac troponin (cTn) on myofilament function i...

Contractile Function of the Human Myocardium Impact of Troponin Phosphorylation

s Zhang P, Kooij V, Murphy AM, van der Velden J, dos Remedios C, van Eyk JE (2010). Site‐specific quantification of phosphorylated cardiac troponin I by MS‐based multiple reaction monitoring. International symposium on elctro‐ and liquid‐ phase seperation techniques, Baltimore, USA. Kooij V, Piersma SR, Tang KW, Jiménez CR, Zang P, van Eyk JE, Murphy AM, van der Velden J, Stienen GJM (2010)...