Effects of cell differentiation and assay conditions on the UDP-glucuronosyltransferase activity in Caco-2 cells.

Effects of Cell Differentiation and Assay Conditions on the UDP-glucuronosyltransferases Activity in Caco-2 Cells

Effect of Passage Number and Culture Time on the Expression and Activity of Insulin-Degrading Enzyme in Caco-2 Cells

Background: Insulin-degrading enzyme (IDE) is a conserved zinc metallopeptidase. Here, we have evaluated the effect of passage number and culture time on IDE expression and activity in colorectal adenocarcinoma cell line (Caco-2). Methods: Caco-2 cells were cultured with different passage ranges of 5-15, 25-35, 52-63 for 48, 72, and 120 hours. Subsequently, IDE expression and enzyme activity we...

Effects of co-treatment with sulforaphane and autophagy modulators on uridine 5′-diphospho-glucuronosyltransferase 1A isoforms and cytochrome P450 3A4 expression in Caco-2 human colon cancer cells

Sulforaphane (SFN), which is highly enriched in cruciferous vegetables, has been investigated for its cancer chemopreventive properties and ability to induce autophagy. Uridine 5'-diphospho (UDP)-glucuronosyltransferase (UGT)1A induction is one of the mechanisms that is responsible for the cancer chemopreventive activity of SFN. The current study demonstrates that rapamycin may enhance the chem...

The expression of most UDP - glucuronosyltransferases ( UGTs ) is increased significantly during Caco - 2 cell differentiation , while UGT 1 A 6 is highly expressed also in undifferentiated cells

The number of words in the Abstract: 249 The number of words in the Introduction (including refs.): 714 The number of words in the Discussion (including refs.): 1245 Abbreviations: UGT, UDP-glucuronosyltransferase; Caco-2, human intestinal cell line derived from colonic cancer; (q)RT-PCR, (quantitative) reverse transcriptase-polymerase chain reaction This article has not been copyedited and for...

Effects of hydrogen peroxide-induced oxidative stress on the pattern of pro-apoptotic and anti-apoptotic genes expression during PC12 cells differentiation

Background and Objective: In neurodegenerative disorders, oxidative stress mediated by reactive oxygen species is strongly associated with increased neuronal damages that lead to apoptosis. Pro-apoptotic and anti-apoptotic gene expressions were changed during cell differentiation that affect cell viability and differentiation. This study was conducted to determine the effects of hydrogen peroxi...