Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

Biochemical characterization of an L-Xylulose reductase from Neurospora crassa.

An l-xylulose reductase identified from the genome sequence of the filamentous fungus Neurospora crassa was heterologously expressed in Escherichia coli as a His(6) tag fusion protein, purified, and characterized. The enzyme may be used in the production of xylitol from the major pentose components of hemicellulosic waste, d-xylose and l-arabinose.

Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis

Xylose reductase (XR) is the first enzyme in D: -xylose metabolism, catalyzing the reduction of D: -xylose to xylitol. Formation of XR in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose. This is one reason why glucose is not a suitable co-substrate for cell growth in i...

Xylitol production by Saccharomyces cerevisiae overexpressing different xylose reductases using non-detoxified hemicellulosic hydrolysate of corncob

Xylitol production was compared in fed batch fermentation by Saccharomyces cerevisiae strains overexpressing xylose reductase (XR) genes from Candida tropicalis, Pichia stipitis, Neurospora crassa, and an endogenous gene GRE3. The gene encoding a xylose specific transporter (SUT1) from P. stipitis was cloned to improve xylose transport and fed batch fermentation was used with glucose as a cosub...

Nikhil Nair and Huimin Zhao crassa Neurospora l - Xylulose Reductase from Biochemical Characterization

Chemical Activation of Ascospore Germination in Neurospora crassa.

In the red bread mold Neurospora crassa as in other pyrenomycetes the sexual spores, the ascospores, germinate only after the application of heat (Shear and Dodge, 1927; Goddard, 1935, 1938, 1939). When treated at a temperature of from 50 to 60 C for periods varying from 10 to 60 minutes the spores will germinate up to 100 per cent. Germination may occur without this treatment, the percentage d...