Mechanisms of lysosomal enzyme release from human polymorphonuclear leukocytes. Effects of phorbol myristate acetate
نویسندگان
چکیده
PMA enhanced release of the azurophil granule enzyme, beta-glucuronidase, as well as lysozyme, from cytochalasin B-treated PMN's exposed to either zymosan particles or C5a. PMA was active at nanomolar concentrations, was not toxic to the cells, and was most effective when present for brief durations (0-1 min) before exposure of the cells to the stimuli. Beta-glucuronidase was not released in significant amounts from PMN's exposed to PMA alone, in the absence of stimuli such as zymosan or C5a. In contrast, only the specific granule enzyme, lysozyme, was released from unstimulated cells. Electron micrographs of cells exposed to PMA revealed an increase in the number of visible cytoplasmic microtubules as compared to control cells. Enhancement of lysosomal enzyme (beta-glucuronidase) release by PMA appears to be independent of effects on release of specific granule enzymes (lysozyme), but rather is likely due to PMA-induced elevations of cellular cGMP.
منابع مشابه
Phenylarsine oxide and ethanol prevent cell death of porcine polymorphonuclear leucocytes induced by phorbol myristate acetate.
In this study, we report that phenylarsine oxide and ethanol, both of which suppress a number of polymorphonuclear leucocyte functions including superoxide production, prevented the phorbol myristate acetate-induced cell death in a dose-dependent manner. These reagents had an inhibitory effect even after polymorphonuclear leucocytes were stimulated to produce superoxide by treatment with phorbo...
متن کاملPertussis toxin inhibition of chemotactic factor-induced calcium mobilization and function in human polymorphonuclear leukocytes
Chemotactic factors stimulate a rapid increase in the cytosolic concentration of intracellular calcium ions ([Ca2+]in) in human polymorphonuclear leukocytes (PMNL), which may be an event that is critical to the expression of chemotaxis and other PMNL functions. Treatment of PMNL with pertussis toxin catalyzes ADP-ribosylation of a protein similar or identical to the inhibiting regulatory protei...
متن کاملExtracellular release of antimicrobial defensins by human polymorphonuclear leukocytes.
Human polymorphonuclear leukocytes (PMN) contain three antimicrobial and cytotoxic peptides which belong to a family of mammalian granulocyte peptides named defensins. To determine their potential availability for extracellular microbicidal or cytotoxic events, we quantified the extracellular release of defensins after stimulation of human PMN with phorbol myristate acetate and opsonized zymosa...
متن کاملChemoattractant - Mediated Stimulation of the Respiratory Burst in Human Polymorphonuclear Leukocytes May Require Appearance
Low doses of aliphatic alcohols produce divergent effects on the function of chemoattractant receptors on human polymorphonuclear leukocytes (PMNs) since they enhance chemotaxis but inhibit stimulation of superoxide production by chemoattractants. As such. alcohols can provide useful pharmacologic tools to probe the mechanisms of stimulus-response coupling in leukocytes. A role for protein kina...
متن کاملChemoattractant - Mediated Stimulation of the Respiratory Burst in Human Polymorphonuclear Leukocytes May Require Appearance of Protein Kinase
Low doses of aliphatic alcohols produce divergent effects on the function of chemoattractant receptors on human polymorphonuclear leukocytes (PMNs) since they enhance chemotaxis but inhibit stimulation of superoxide production by chemoattractants. As such. alcohols can provide useful pharmacologic tools to probe the mechanisms of stimulus-response coupling in leukocytes. A role for protein kina...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 66 شماره
صفحات -
تاریخ انتشار 1975