A new series of CAT expression vectors.

Construction of New Genetic Tools as Alternatives for Protein Overexpression in Escherichia coli and Pseudomonas aeruginosa

Background: Pseudomonas protein expression in E. coli is known to be a setback due to signifi cant genetic variation and absence of several genetic elements in E. coli for regulation and activation of Pseudomonas proteins. Modifi cations in promoter/repressor system and shuttle plasmid maintenance have made the expression of stable and active Pseudomonas protein possible in bot...

Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors.

Expression of chloramphenicol acetyltransferase (cat) by suitable vectors in chloroplasts of cultured tobacco cells, delivered by high-velocity microprojectiles, is reported here. Several chloroplast expression vectors containing bacterial cat genes, placed under the control of either psbA promoter region from pea (pHD series) or rbcL promoter region from maize (pAC series) have been used in th...

A modified CAT expression vector with convenient cloning sites.

The chloramphenicol acetyl transferase (CAT) gene is widely used as a reporter gene to analyse promoters and other gene regulatory elements. Cloning of a DNA fragment into a CAT vector such as pSVO CAT (1) usually involves blunt-end ligation with no control over the orientation of the insert. This is because the first generation CAT vectors lack convenient multiple restriction sites immediately...

A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells.

The pJfi vector series has been designed to allow easy insertion and subsequent expression of exogenous genes in a wide variety of mammalian cells. The vectors share a common structure of a mammalian transcription unit composed of a promoter flanked 3' by a polyhnker, an intron, and a transcriptional termination signal which is linked to a pBR 322 derived backbone as shown. Each of the six vect...

Construction and characterization of regulated L-arabinose-inducible broad host range expression vectors in Xanthomonas.

Several versions of broad host range (BHR), L-arabinose-inducible expression vectors were constructed. These expression vectors were based on a high copy number BHR pBBR1MCS-4 replicon that could replicate in both enteric and non-enteric Gram-negative bacteria. Two versions of expression cassettes containing multiple cloning sites either with or without a ribosome binding site were placed under...

The Role of Bacterial Vectors in the Expression of Human IFN-y Gene