RNA-sequencing Reveals Global Transcriptomic Changes in Nicotiana tabacum Responding to Topping and Treatment of Axillary-shoot Control Chemicals
نویسندگان
چکیده
Removal of terminal buds (topping) and control of the formation of axillary shoots (suckers) are common agronomic practices that significantly impact the yield and quality of various crop plants. Application of chemicals (suckercides) to plants following topping is an effective method for sucker control. However, our current knowledge of the influence of topping, and subsequent suckercide applications, to gene expression is limited. We analyzed the differential gene expression using RNA-sequencing in tobacco (Nicotiana tabacum) that are topped, or treated after topping by two different suckercides, the contact-localized-systemic, Flupro(®) (FP), and contact, Off-Shoot-T(®). Among the differentially expressed genes (DEGs), 179 were identified as common to all three conditions. DEGs, largely related to wounding, phytohormone metabolism and secondary metabolite biosynthesis, exhibited significant upregulation following topping, and downregulation after suckercide treatments. DEGs related to photosynthetic processes were repressed following topping and suckercide treatments. Moreover, topping and FP-treatment affect the expression of auxin and cytokinin signaling pathway genes that are possibly involved in axillary shoot formation. Our results provide insights into the global change of plant gene expression in response to topping and suckercide treatments. The regulatory elements of topping-inducible genes are potentially useful for the development of a chemical-free sucker control system.
منابع مشابه
RNA-Sequencing Reveals Global Transcriptomic Changes in <em>Nicotiana tabacum</em> Responding to Topping and Treatment of Axillary-Shoot Control Chemicals
متن کامل
Effects of Topping and Suckericide on Leaf Quality of Tobacco (Nicotiana Tabacum)
Topping stage of tobacco is a key time for development of agriculture measures to promote the quality of leaves. In order to investigating influence of topping timing and suckericide content on tobacco leaf, a factorial experiment on the basis of RCB design with 3 replications was conducted in 2011 at the Tobacco Research Farm of Isfahan. Treatments were including topping time (T1: t...
متن کاملEffects of Topping and Suckericide on Leaf Quality of Tobacco (Nicotiana Tabacum)
Topping stage of tobacco is a key time for development of agriculture measures to promote the quality of leaves. In order to investigating influence of topping timing and suckericide content on tobacco leaf, a factorial experiment on the basis of RCB design with 3 replications was conducted in 2011 at the Tobacco Research Farm of Isfahan. Treatments were including topping time (T1: t...
متن کاملRNA Sequencing Analysis Reveals Transcriptomic Variations in Tobacco (Nicotiana tabacum) Leaves Affected by Climate, Soil, and Tillage Factors
The growth and development of plants are sensitive to their surroundings. Although numerous studies have analyzed plant transcriptomic variation, few have quantified the effect of combinations of factors or identified factor-specific effects. In this study, we performed RNA sequencing (RNA-seq) analysis on tobacco leaves derived from 10 treatment combinations of three groups of ecological facto...
متن کاملFertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana
The Fertilization Independent Endosperm (FIE) gene is required to restrict endosperm development without fertilization, and it represses flowering during embryo and seedling development in Arabidopsis thaliana However, the regulatory mechanism of the FIE gene in postembryonic shoot development is not well understood. Silencing of Nicotiana benthamiana homologues of the FIE gene, NbFIE1 and NbFI...
متن کامل