Supplemental Methods for “The impact of gene expression variation on the robustness and evolvability of a developmental gene regulatory network”
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چکیده
CONTENTS Animal rearing and sample collection . . . . . . . . . . . . . . . . 1 Measurements of larval morphology . . . . . . . . . . . . . . . . . 2 Analyses of larval morphology . . . . . . . . . . . . . . . . . . 2 Estimates of additive genetic variances and covariances . . . . . . . . . . 3 Bayesian estimates of quantitative genetics parameters . . . . . . . . . . 6 Additive genetic variances over development . . . . . . . . . . . . . . 10 Genetic covariances over development . . . . . . . . . . . . . . . . 11 Pre-processing raw bead-level data from DASL . . . . . . . . . . . . . 12 Expression validation . . . . . . . . . . . . . . . . . . . . . 19 Supplemental References . . . . . . . . . . . . . . . . . . . . 22
منابع مشابه
The Impact of Gene Expression Variation on the Robustness and Evolvability of a Developmental Gene Regulatory Network
Regulatory interactions buffer development against genetic and environmental perturbations, but adaptation requires phenotypes to change. We investigated the relationship between robustness and evolvability within the gene regulatory network underlying development of the larval skeleton in the sea urchin Strongylocentrotus purpuratus. We find extensive variation in gene expression in this netwo...
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