Electrochemical Agarose Stamp for Addressable Micropatterning

نویسندگان

  • S. Sekine
  • S. Nakanishi
  • T. Miyake
  • K. Nagamine
  • M. Nishizawa
چکیده

We have developed a hydrogel-based microstamp device that internally contains an electrochemical system for controlled supply of reactive ink to the stamp surface. An agarose stamp was combined with a microelectrode system for the controlled supply of HBrO by an electrochemical reaction. The supplied HBrO will detach the heparin layer pre-coated on substrates and allow selective adsorption of proteins to the exposed substrate surfaces. Especially, by combination the stamp with a microelectrode array, we demonstrate the spatially addressable micropatterning.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

deposition using an agarose stamp

Electrochemical wet stam an agarose stamp. The ag depressions using its prop ential electrochemical de gel stamp behaves as a cu Furthermore, it simultane tures complementary of t the fabricated microstruc

متن کامل

Multicolour micropatterning of thin films of dry gels.

Micropatterning of surfaces with several chemicals at different spatial locations usually requires multiple stamping and registration steps. Here, we describe an experimental method based on reaction-diffusion phenomena that allows for simultaneous micropatterning of a substrate with several coloured chemicals. In this method, called wet stamping (WETS), aqueous solutions of two or more inorgan...

متن کامل

Micropatterned agarose gels for stamping arrays of proteins and gradients of proteins.

We describe a method for repetitive and rapid formation of planar microarrays and gradients of proteins using patterned agarose stamps. It demonstrates: (i) micropatterning of agarose gels with feature sizes as small as 2 microm; (ii) inking of posts (diameter 50-1000 microm) on patterned agarose stamps with one or multiple (here, eight) proteins and repetitive stamping of patterns (>100 times ...

متن کامل

Microcontact Peeling as a New Method for Cell Micropatterning

Micropatterning is becoming a powerful tool for studying morphogenetic and differentiation processes of cells. Here we describe a new micropatterning technique, which we refer to as microcontact peeling. Polydimethylsiloxane (PDMS) substrates were treated with oxygen plasma, and the resulting hydrophilic layer of the surface was locally peeled off through direct contact with a peeling stamp mad...

متن کامل

Designing Electroactive Biointerface for Spatiotemporal Control of Cell Attachment and Release

In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2010