Effects of Centrifugation on Equine Spermatozoa Immediately and after Cooling for 24 Hours
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چکیده
.................................................................................................vii CHAPTER 1: INTRODUCTION..........................................................................1 CHAPTER 2: LITERATURE REVIEW.................................................................3 2.1. Stallion Spermatozoa........................................................................3 2.1.1. Spermatozoal Structure and Function.......................................3 2.1.2. Spermatozoal Metabolism......................................................6 2.1.3. Reactive Oxygen Species (ROS).............................................7 2.2. Processing of Fresh Cooled Semen.....................................................9 2.2.1. Seminal Plasma...................................................................9 2.2.2. Seminal Plasma Dilution........................................................10 2.2.3. Centrifugation and Sperm Recovery Rate................................11 2.2.4. Passive Cooling..................................................................14 2.3. Semen Evaluation...........................................................................14 2.3.1. Motility..............................................................................16 2.3.2. Concentration.....................................................................17 2.3.3. Morphology........................................................................17 2.3.4. Fluorescent Probes..............................................................18 CHAPTER 3: CENTRIFUGATION HAS MINIMAL EFFECTS ON MOTILITY, VIABILITY AND ACROSOME INTEGRITY OF EQUINE SPERMATOZOA IMMEDIATELY AND AFTER COOLING FOR 24 HOURS 3.
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