Printed in Great Britain Ribonucleic Acid-Deoxyribonucleic Acid Hybridization as a Second-Order Reaction By
نویسنده
چکیده
The formation of specific RNA-DNA hybrid molecules has been widely used to study relationships between RNA and DNA sequences. Although this reaction probably involves mechanisms similar to those of DNA renaturation, the analysis of its kinetics is more complicated because the relative concentrations of complementary RNA and DNA sequences are often unknown. Moreover during hybridization the DNA is often immobilized to prevent renaturation. Nygaard & Hall (1964) studied the formation of ribonuclease-resistant RNA-DNA duplexes in solution and concluded that the initial reaction rate
منابع مشابه
Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r.
An increase in the rate of synthesis of ara-specific messenger ribonucleic acid as measured by deoxyribonucleic acid-ribonucleic acid hybridization has been detected in the induced wild-type (ara(+)) strain of Escherichia coli B/r as compared with the uninduced control, thus providing evidence that regulation of the positively controlled l-arabinose operon is at the level of transcription.
متن کاملThe role of deoxyribonucleic acid in ribonucleic acid synthesis. I. The purification and properties of ribonucleic acid polymerase.
It has become evident that several enzymatic reactions exist which lead to the incorporation of the mononucleotide moiety of the ribonucleoside triphosphates into ribonucleic acid (1). The first of these enzymes to be characterized catalyzes the addition of cytosine 5’-phosphate and adenosine 5’-phosphate to the nucleoside terminal end of soluble ribonucleic acid (2,3). The product of this reac...
متن کاملRibonucleic acid-deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide.
Hybridization in 6xSSC (SSC, 0.15m-sodium chloride-0.015m-sodium citrate) at 66 degrees C was compared with hybridization in formamide-6xSSC (1:1, v/v) at 35 degrees C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridizati...
متن کاملThe Isolation and Characterization of Adenosine Monophosphate-rich Polynucleotides Synthesized by Soybean Hypocotyl Cells: Their Relation to Messenger Ribonucleic Acid.
Plant ribonucleic acids which have high adenosine monophosphate concentrations were studied. Purified deoxyribonucleic acid-like ribonucleic acid and tenaciously bound ribonucleic acid fractions both contained poly-adenosine monophosphate sequences (those from the latter being longer than those from the former); without these poly-adenosine monophosphate sequences their base compositions were t...
متن کاملCharacteristics of the syrian hamster ribonucleic acid present in cells transformed by polyoma, simian virus 40, or adenovirus 12.
Ribonucleic acids of known specific activities were separately prepared from hamster cells transformed by polyoma, simian virus 40 (SV40), and adenovirus 12. When increasing amounts of these labeled ribonucleic acids were added to nitrocellulose filters containing immobilized (14)C-hamster deoxyribonucleic acid (DNA), about 5% of the DNA sites were saturated in each case. An additive saturation...
متن کامل