Enzymatic Synthesis of 1,6-Digalloylglucose from ß-Glucogallin by ß-Glucogallin: ß-Glucogallin 6-0-Galloyltransferase from Oak Leaves
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چکیده
ß-Glucogallin (1 -O-galloyl-ß-D-glucopyranose), 1,6-Di-O-galloyl-ß-D-glucopyranose, Acyltransferase, Gallotannin Biosynthesis, Quercus robur Cell-free extracts from oak (Quercus robur) leaves catalyze the transfer of the galloyl-moiety of ß-glucogallin (1-O-galloyl-ß-D-glucopyranose) specifically to the 6-position of the same compound, yielding 1,6-di-O-galloyl-ß-D-glucopyranose, an intermediate of gallotannin biosynthesis, ßGlucogallin thus functions as both donor and acceptor molecule in this reaction. The partial purification and some general properties of this new acyltransferase are reported.
منابع مشابه
Biosynthesis of Gallotannins. ß-Glucogallin-Dependent Galloylation of 1,6-DigaIloylglucose to 1,2,6-Trigalloylglucose
An enzyme from leaves o f sumach ( Rhus typhina) was partially purified that catalyzes the ß-glucogallin (l-O-galloylglucose)-dependent galloylation o f 1,6-digalloylglucose, thus form ing 1,2,6-trigalloylglucose and free glucose. This acyltransferase had a molecular weight o f ca. 750,000 and a pH optimum at 5 .0 -5 .5 . Besides ß-glucogallin (Km 3.9 m M ) , also related 1-O-phenylcarboxylglu...
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