Caries-free subjects have high levels of urease and arginine deiminase activity

نویسندگان

  • Evelyn REYES
  • Javier MARTIN
  • Gustavo MONCADA
  • Miguel NEIRA
  • Patricia PALMA
  • Valeria GORDAN
  • Juan Fernando OYARZO
  • Ismael YEVENES
چکیده

OBJECTIVES This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. MATERIAL AND METHODS Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. RESULTS The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. CONCLUSIONS Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects.

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عنوان ژورنال:

دوره 22  شماره 

صفحات  -

تاریخ انتشار 2014