Observation of curative effect regarding corneal cross-linking treatment of riboflavin combined with 440 nm blue-light cornea for fungal keratitis

To observe and analyze the curative effects of the treatment of riboflavin combined with 440 nm blue-light corneal cross-linking (CXL) treatment for fungal keratitis via a rabbit model experiment. A model of rabbit fungal keratitis was used through anterior stromal injection in which 24 rabbits were randomly divided into blank control, 360 nm group, and 440 nm group. Both groups underwent CXL treatment. Ultraviolet radiation A (365 nm) and blue-light (440 nm) irradiations were performed for 30 min after 30 min of 0.1% riboflavin eye drops used in 360 nm and 440 nm groups, respectively. Slit-lamp observation, photography of the cornea, calculation of ulcer area, and comparison of examination under confocal microscope were performed for all rabbits on the first, third, and seventh days after treatment. No statistical significance was observed between all groups on the first day after treatment (P>0.05). In 3 days after surgery, the cornea ulcer area decreased by 35.65% in the 360 group (P=0.012) and 37.94% in the 440 group (P=0.015), thus the two groups showing no statistical significance (P=0.88). In 7 days after surgery, the area vanished by 59.02% in the 360 group (P=0.00) and 55.29% in the 440 group (P=0.001), thus the two groups showing statistical significance (P=0.75). Riboflavin with 440 nm blue-light irradiation had a positive effect on treatment for fungal keratitis.