Laccase Immobilization by Chelated Metal Ion Coordination Chemistry
نویسندگان
چکیده
In this work, amidoxime polyacrylonitrile (AOPAN) nanofibrous membrane was prepared by a reaction between PAN nanofibers and hydroxylamine hydrochloride. The AOPAN nanofibrous membranes were used for four metal ions (Fe, Cu, Ni, Cd) chelation under different conditions. Further, the competition of different metal ions coordinating with AOPAN nanofibrous membrane was also studied. The AOPAN chelated with individual metal ion (Fe, Cu, Ni, Cd) and also the four mixed metal ions were further used for laccase (Lac) immobilization. Compared with free laccase, the immobilized laccase showed better resistance to pH and temperature changes as well as improved storage stability. Among the four individual metal ion chelated membranes, the stability of the immobilized enzymes generally followed the order as Fe–AOPAN–Lac > Cu–AOPAN–Lac > Ni–AOPAN–Lac > Cd–AOPAN–Lac. In addition, the immobilized enzyme on the carrier of AOPAN chelated with four mixed metal ions showed the best properties.
منابع مشابه
Bioinspired Immobilization of Glycerol Dehydrogenase by Metal Ion-Chelated Polyethyleneimines as Artificial Polypeptides
In this study, a novel, simple and generally applicable strategy for multimeric oxidoreductase immobilization with multi-levels interactions was developed and involved activity and stability enhancements. Linear polyethyleneimines (PEIs) are flexible cationic polymers with molecular weights that span a wide range and are suitable biomimic polypeptides for biocompatible frameworks for enzyme imm...
متن کاملFunctionalized magnetic mesoporous silica nanoparticles: fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth.
A simple and highly efficient protocol using magnetic mesoporous silica nanoparticles (MMSNPs) with metal affinity ligands was developed to directly capture laccase from Trametes versicolor fermentation broth. The Cu(2+)-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu(2+)) with pore sizes ranging from 3.6 to 27.1 nm exhibited size selectivity on laccase capture from the fermentatio...
متن کاملThe "unique" metal-binding properties of metalloenzymes.
(1) The theme of the survey is that metalloenzymes represent rather unique' metal complexes when compared to the simpler system generally studied by coordination chemists. This theme is introduced by a brief presentation of the historical development of the metalloenzyme field, particularly the contributions of 0. Warburg and D. Keilin. (2) Various types of metal ion—protein interactions and th...
متن کاملEnhancement of stability of glucose oxidase by immobilization onto metal ion-chelated poly(N-vinyl imidazole) hydrogels.
Poly(N-vinylimidazole), PVIm, gels were prepared by gamma-irradiation polymerization of N-vinylimidazole in aqueous solutions as an affinity gel for glucose oxidase (GOx). These affinity gels with a water swelling ratio of 1800% for plain polymeric gel and between 30-80% for Cu(II) and Co(II)-chelated gels at pH 6.0 in phosphate buffer were used in the GOx adsorption studies. Maximum metal ion ...
متن کاملOne and Two Dimensional Pulsed Electron Paramagnetic Resonance Studies of in vivo Vanadyl Coordination in Rat Kidney
The biological fate of a chelated vanadium source is investigated by/n vivo spectroscopic methods to elucidate the chemical form in which the metal ion is accumulated. A pulsed electron paramagnetic resonance study of vanadyl ions in kidney tissue, taken from rats previously treated with bis(ethylmaltolato)oxovanadium(IV) (BEOV) in drinking water, is presented. A combined approach using stimula...
متن کامل