Equilibrium dialysis and cell binding studies on Bandeiraea simplicifolia lectin.

An immunochemical study of the combining sites of the second lectin isolated from Bandeiraea simplicifolia (BS II).

Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography.

Bandeiraea simplicifolia plant seeds contain a family of five alpha-D-galactopyranosyl-binding isolectins (BS I-A4, A3B, A2B2, AB3, B4) and N-acetyl-D-glucosamine-binding lectin (BS II). After Pi/NaCl extraction and ammonium sulfate fractionation BS II is specifically adsorbed onto p-aminobenzyl-1-thio-N-acetyl-beta-D-glucosaminide-succinylaminohexylaminyl--Sepharose-4B. The BS I isolectins pas...

Serological studies on an alpha-D-galactosyl-binding lectin isolated from Bandeiraea simplicifolia seeds.

Abstruct. The serological characteristics of a highly purified a-D-galactosyl-binding lectin, isolated from extracts of Buntfeiraen siniplicifoliu seeds, are described. These studies show that the lectin preferentially agglutinates group B red cells and in addition has the capacity to distinguish between group A, and group A, erythrocytes. Since the lectin is not absolutely specific for group B...

An -N-acetyl-D-glycosamine binding lectin from Bandeiraea simplicifolia seeds.

An cY-n-galactopyranosyl binding lectin (1) was recently isolated and characterized from the seed extracts ofBandeiraea simplicifolia. While pursuing further studies on this lectin, a second protein capable of interacting with p-azophenyl 2-acetamido-2deoxy-aand -p-n-glucopyranoside BSA’ conjugates was discovered. The new lectin, isolated from the same plant seed by affinity chromatography, sho...

Inhibition of Ehrlich ascites tumor cell growth by Griffonia simplicifolia I lectin in vivo.

Griffonia (Bandeiraea) simplicifolia I (GS I) seeds contain a family of alpha-D-galactopyranosyl-binding isolectins which strongly agglutinate Ehrlich ascites tumor cells due to the presence of this determinant sugar on their cell surface glycoproteins. Administration of GS I lectin (100 micrograms/day or 300 micrograms on alternate days, i.p.) inhibits the growth of Ehrlich tumor cells in vivo...