The effect of aldosterone of glycolysis in the urinary bladder of the toad.
نویسندگان
چکیده
Prolonged incubation with aldosterone causes an increase in the rate of sodium transport and in the rate of glycolysis in the urinary bladder of the toad (Bufo marinus) in vitro. When sodium transport was inhibited by ouabain after the effect of aldosterone was manifest, the rate of sodium transport as well as the rate of glycolysis fell to the same low value in aldosterone-treated tissue and in paired control tissue that had been incubated without hormone. When bladders were made anaerobic after the aldosterone effect was manifest, short circuit current fell in both preparations, but remained significantly higher in aldosterone-treated tissue than in paired control tissue. During anaerobiosis, the rate of lactate production was higher in aldosteronetreated tissue than in paired anaerobic control tissue. Thus the effect of aldosterone on glycolysis appears to be related to its effect on sodium transport and does not depend entirely upon oxidative metabolism. Measurement of the concentration of glycolytic intermediates and cofactors in tissue incubated with aldosterone indicated activation of phosphofructotiase and pyruvate ease. There was no change in the concentration of adenosine mono-, di-, or triphosphate, or inorganic phosphate, but there was a fall in creatine phosphate and a rise in the concentration of creatine in aldosterone-treated tissue. This pattern is interpreted as indicating that the stimulation of sodium transport by aldosterone (or an aldosterone-induced protein) is a direct effect on sodium transport and not secondary to an effect of the hormone on energy production.
منابع مشابه
Aldosterone increases the apical Na+ permeability of toad bladder by two different mechanisms.
The aldosterone-induced augmentation of Na+ transport in toad bladder was analyzed by comparing the hormonal actions on the transepithelial short-circuit current and on the amiloride-sensitive 22Na+ uptake in isolated membrane vesicles. Incubating bladders with 0.5 microM aldosterone for 3 hr evoked more than a 2-fold increase of the short-circuit current (because of the activation or insertion...
متن کاملAldosterone and insulin effects on driving force of Na+ pump in toad bladder.
Both aldosterone and insulin increase active Na+ transport across the urinary bladder of the toad. Recent data have provided further support to the concept that aldosterone acts primarily to increase Na+ entry from the mucosal medium into the transporting cells, whereas insulin acts to increase active Na+ extrusion into the serosal medium. To examine this concept further, the driving force (E(N...
متن کاملSteroid-induced protein synthesis in giant-toad (Bufo marinus) urinary bladders. Correlation with natriferic activity.
We have identified a group of proteins (Mr approximately 70 000-80 000; pI approximately 5.5-6.0) in giant-toad (Bufo marinus) urinary bladders whose synthesis appears to be related to aldosterone-stimulated Na+ transport. Spironolactone, a specific mineralocorticoid antagonist in renal epithelia, inhibits the synthesis of these proteins as well as the natriferic effect of the hormone. Since a ...
متن کاملGlyoxylate cycle in toad urinary bladder: possible stimulation by aldosterone.
A homogenate of the toad urinary bladder epithelial cell layer has the two enzymatic activities unique to the glyoxylate cycle--isocitrate lyase (threo-Ds-isocitrate glyoxylate-lyase, EC 4.1.3.1) and malate synthase [L-malate glyoxylate-lyase (CA-acetylating), EC 4.1.3.2]--as well as the capacity to carry out CN-insensitive palmitoyl-CoA oxidation. When tissue is incubated in the presence of a ...
متن کاملAmiloride-sensitive trypsinization of apical sodium channels. Analysis of hormonal regulation of sodium transport in toad bladder
Incubation of the mucosal surface of the toad urinary bladder with trypsin (1 mg/ml) irreversibly decreased the short-circuit current to 50% of the initial value. This decrease was accompanied by a proportionate decrease in apical Na permeability, estimated from the change in amiloride-sensitive resistance in depolarized preparations. In contrast, the paracellular resistance was unaffected by t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 244 12 شماره
صفحات -
تاریخ انتشار 1969