Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): downregulation of specific cytokine secretion (IL-9 and IL-10) correlates with poor mastocytosis and chronic survival of adult

(1993) Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): downregulation of specific cytokine secretion (IL-9 and IL-10) correlates with poor mastocytosis and chronic survival of adult worms. The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. A note on versions: The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. Immunological relationships during primary infection with Heligmosomoides polygyrus (Nernatospiroides dubius): downregulation of specific cytokine secretion (IL-9 and IL-10) correlates with poor mastocytosis and chronic survival of adult worms SUMMARY Mice were infected either with Trichinella spiralis (day U) , Heligmosomoides polygyrus (day-14) or concurrently with both species and were killed in groups, together with nai've control mice, on 2 occasions (day 8 and 15 post injection with T. spiralis, corresponding to days 22 and 29 p.i. with H. polygyrus). The expulsion of T. spiralis was slowed significantly in concurrently infected mice and this was ussociated with a reduced mastocytosis and lower serum mucosal must cell protease levels. Mesenteric lymph node (M L N) lymphocytes from all three experimentul groups secreted IL-3 and IL-4 in copious amounts when stimulated in vitro by Concanavalin A (Con-A), hut the secretion o f high levels of IL-9 und IL-10 was essentially conjned to mice infected with T. spiralis alone. It is suggested that adult H. polygyrus selectively modulate cytokine secretion by Th2 cells within the M L N during infection and that this is brought about as a direct consequence of the mechanism employed by H. polygyrus to depress mucosal inflammatory responses in order to facilitate its own survival.