The Effects of Topical Fibrin Glue and Epidermal Growth Factor (egf) on the Colon Anastomosis Healing Process – a Comparative Experimental Study

In this experimental study on small animals (Wistar) we evaluated the effectiveness of local protection methods (application of fibrin glue) and combined fibrin glue and EGF on colon anastomosis healing – prevention of dehiscence. Ninety experimental animals, with segmental left colonic resection in general anesthesia, were divided into 3 groups, each of 30 animals. In the control group, termino-terminal anastomosis with single-layer alternate suture was performed after segmental resection. In the second and third group, anastomosis protection was performed with extraluminal application of fibrin glue (coating) and combined fibrin glue and EGF. In the postoperative course of 21 days, 3 control animals died (10%) due to acute diffuse peritonitis caused by anastomosis dehiscence, in the group with combined fibrin glue and EGF 1 animal died (3.3%), while in the group with extraluminally applied fibrin glue only, all animals survived. The results demonstrate that local protective measures in colonic anastomosis yield better results; fibrin glue application and combined fibrin glue and EGF demonstrated similar results.