Differential Labeling of Reversible Protein-Oxidation and S-Palmitoylation Using the Biotin-Switch Assay
نویسندگان
چکیده
immediate response of eukaryotic cells to oxidative. PARP-1 and PARP-2 display catalytic activity by contact with DNA-strand breaks and are involved in DNA base-excision repair and other repair pathways. Recent studies suggest a link between DNA damage-induced poly(ADP-ribosyl)ation and all tissues at varying levels, with the highest activity in mammalian testis, spleen and thymus. in particular, the poly(ADP-ribosyl)ation is, therefore, pivotal in the maintenance of genomic integrity as well as in the cellular responses to DNA damage. the aim of the present research has been to determine the PARP activity and to study protein expression in the testis of frog, morphological and molecular identified as Pelophylax bergeri, of three different polluted sites and one control site. Antioxidative Glutathione Stransferase activity has been also determined in order to correlate the poly(ADP)ribosylation with the oxidative stress. Moreover the biochemical data have been integrated with morphological evaluation, to assess the health status of the gonadal tissue.
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