Biochemical and biological properties of methotrexate analogs containing D-glutamic acid or D-erythro, threo-4-fluoroglutamic acid.
نویسندگان
چکیده
on the B-helix transition of poly-L-lysine in sodium 18. Watanabe S and Saito T, A CD study of the role of dodecyl sulfate solution. Biopolymers 14: 1841-1846. metal ions in the conformation of poly(L-Lysine). 1975. Biopolymers 26: 625-632, 1987. 17. Sarker PK and Doty P, The optical rotatory properties 19. Davidson B and Fasman GD, The conformational of the P-configuration in polypeptides and proteins. transitions of uncharged poly-L-lysine. (Y Helix-random Proc Natl Acad Sci USA 55: 981-989, 1966. coil-8 structure. Biochemistry 6: 16161629. 1967.
منابع مشابه
L-erythro- AND D,L-three-y-FLUOROMETHOTREXATE WITH HUMAN LEUKEMIA CELL DIHYDROFOLATE REDUCTASE
Gamma-fluoromethotrexate (FMTX) is a poorly glutamylated mimic of the anti-cancer drug methotrexate (MTX) which is useful in studies of the roles of MTX poly-y-glutamates. A second chiral center occurs at C-4 of the 4-fluoroglutamate used to synthesize FMTX and, as a consequence, FMTX occurs as both D,L-eryrhro and D,L-the0 diastereomers. The interaction of both diastereomers with intracellular...
متن کاملInteraction of L-threo and L-erythro isomers of 3-fluoroglutamate with glutamate decarboxylase from Escherichia coli.
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis of the unstable intermediate enamine. The ...
متن کاملDiastereomer-dependent substrate reduction properties of a dinitrogenase containing 1-fluorohomocitrate in the iron-molybdenum cofactor.
In vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of dinitrogenase using homocitrate and its analogs allows the formation of modified forms of FeMo-co that show altered substrate specificities (N2, acetylene, cyanide, or proton reduction) of nitrogenase [reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolyzing), EC 1.18.6.1]. The (1R,2S)-threo- and (1S,2S)-erythro-fluorinated di...
متن کاملComparison Studies on Complexation of Molybdenum(VI) with Aspartic Acid And Glutamic Acid in Different Dielectric Constants1
Equilibria of the reaction of molybdenum(VI) with L-aspartic acid (Asp) and L- Glutamic acid(Glu) have been studied by spectrophotometric measurements in mixed solvent systems at an ionicstrength of 0.2 mol/dm3 sodium perchlorate, employed (15, 20, 25, 30± 0.1°C) at pH ranges of 3.2to 5.5 with a high ratio of ligand to metal. The stability constants of the complexes and theresulting free energy...
متن کاملTotal synthesis of stereospecific sphingosine and ceramide.
A small-scale synthesis of the four sphingosine stereoisomers (d-erythro, l-erythro, d-threo, and l-threo) and lignoceroyl d- and l-erythro-sphingosines, which is suitable for synthesis of tritium-labeled compounds, is described. Ethyl dl-erythro-2-acetamino-3-hydroxy-4t-octadecenoate was esterified with l(+)-acetylmandeloyl chloride and the two diastereomers obtained were separated from each o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biochemical pharmacology
دوره 42 12 شماره
صفحات -
تاریخ انتشار 1991