Integration of 18O labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins.

نویسندگان

  • Jinshan Wang
  • Peter Gutierrez
  • Nathan Edwards
  • Catherine Fenselau
چکیده

Forward and reverse (18)O labeling are integrated with solution isoelectric focusing and capillary LC-tandem mass spectrometry to evaluate a new strategy for quantitative proteomics and to study abundance changes in mitochondrial proteins associated with drug resistance in MCF-7 human cancer cells. Galectin-3 binding protein, which is involved in apoptosis, was detected only in the resistant cell line, as a result of reverse labeling. Among 278 proteins identified, 12 were detected with abundances altered at least 2-fold.

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عنوان ژورنال:
  • Journal of proteome research

دوره 6 12  شماره 

صفحات  -

تاریخ انتشار 2007