An Essential Role for Talin during M 2-mediated Phagocytosis
نویسندگان
چکیده
The cytoskeletal, actin-binding protein talin has been previously implicated in phagocytosis in Dictyostelium discoideum and mammalian phagocytes. However, its mechanism of action during internalization is not understood. Our data confirm that endogenous talin can occasionally be found at phagosomes forming around IgGand C3bi-opsonized red blood cells in macrophages. Remarkably, talin knockdown specifically abrogates uptake through complement receptor 3 (CR3, CD11b/CD18, M 2 integrin) and not through the Fc receptor. We show that talin physically interacts with CR3/ M 2 and that this interaction involves the talin head domain and residues W747 and F754 in the 2 integrin cytoplasmic domain. The CR3/ M 2–talin head interaction controls not only talin recruitment to forming phagosomes but also CR3/ M 2 binding activity, both in macrophages and transfected fibroblasts. However, the talin head domain alone cannot support phagocytosis. Our results establish for the first time at least two distinct roles for talin during CR3/ M 2mediated phagocytosis, most noticeably activation of the CR3/ M 2 receptor and phagocytic uptake.
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