Multiplex PCR Screening of Y Chromosome Microdeletions in Azoospermic Patients
نویسندگان
چکیده
Objective To develop a multiplex PCR protocol for routine screening of microdeletions on the Y chromosome Methods Five multiplex sets were established and Y chromosome microdeletions screening were carried out in 26 azoospermic men who undertook ICSI and 30 azoospermic men who undertook testicular biopsy. Results In 56 azoospermic men, 5 patients were found with AZFc/DAZ microdeletions, 2 patients were accompanied by AZFc/DAZ and AZFb/RBM1 double microdeletion, and 1 patient had only single sY153 microdeletion. Conclusion The multiplex PCR protocol presented in this study is an easy and reliable method for detecting microdeletions on the Y chromosome. Routine screening for microdeletions on the Y chromosome in azoospermic patients is essential.
منابع مشابه
Frequency of Y Chromosome Microdeletions in Azoospermic and Oligospermic Iranian Infertile Men
Background and Aims: Azoospermia factor (AZF) region of the Y-chromosome has several genes which are responsible for normal spermatogenesis. Microdeletions of these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction. The aim of this study is to determine the frequ...
متن کاملMultiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men
BACKGROUND Y chromosomal microdeletion is an important genetic disorder, which may arise due to intrachromosomal recombination between homologous sequences in the male specific region of the human Y chromosome. It is frequently associated with the quantitative reduction of sperm. The screening for Y chromosomal microdeletions has a great clinical value. OBJECTIVE To develop a sequence tagged ...
متن کاملScreening for Y-chromosome microdeletions in infertile Indian males: utility of simplified multiplex PCR.
BACKGROUND & OBJECTIVE Analysis of the microdeletions in the azoospermia factor (AZF) region of Y chromosome by PCR is an important screening tool in the work-up of infertile males opting for assisted reproductive techniques. In the present study, the Y chromosome microdeletions were analyzed by PCR using primers corresponding to 16 sequence tagged sites (STS) and three genes of the AZF region ...
متن کاملبررسی مولکولی حذفهای کروموزوم Y در نواحی AZF بیماران مبتلا به آزواسپرمی و اولیگواسپرمی غیر انسدادی مراجعهکننده به مرکز ناباروری منتصریه مشهد
Background and Aim: The Y-chromosome azoospermic factor (AZF) regions consist of genes whose specific roles and functions in spermatogenesis and fertility have not been completely clarified. Hence, recognition of the association between AZF microdeletions and male infertility has suggestions for the diagnosis, treatment, and genetic counseling. The main objective of the present study was invest...
متن کاملPrevalence and patterns of Y chromosome microdeletion in infertile men with azoospermia and oligzoospermia in Northeast China
BACKGROUND In some cases infertile men showed small deletions of specific genes in the Y chromosome. It had been confirmed, these deleted genes are greatly associated with spermatogenic failure. However, the frequency and the patterns of such microdeletions among infertile men are not clearly clarified. OBJECTIVE We sought to determine the frequency and the patterns of Y chromosome microdelet...
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