φXANES: In vivo imaging of metal-protein coordination environments
نویسندگان
چکیده
We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
منابع مشابه
Erratum: φXANES: In vivo imaging of metal-protein coordination environments
Strains. Wild type C. elegans (strain N2) were obtained from the Caenorhabditis Genetics Center (University of Minnesota), and ftn-2(ok404) and the ferritin null ftn-2(ok404); ftn-1(ok3625) (strain GMC005) have been previously described1. All strains were cultured at 20 °C on standard nematode growth media (NGM) with E. coli (strain OP50)2. Populations were developmentally synchronized via a 3 ...
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