Advances in cassava research in management of Cassava Brown Streak Virus Disease in Uganda

نویسنده

  • Settumba Mukasa
چکیده

Résumé Research Application Summary Advances in cassava research in management of Cassava Brown Streak Virus

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EFFICIENCY OF NON-VECTOR METHODS OF CASSAVA BROWN STREAK VIRUS TRANSMISSION TO SUSCEPTIBLE CASSAVA PLANTS Rwegasira GM*1 and MER Chrissie2

Several etiological and epidemiological studies have been undertaken to determine the disease causal agent and the mechanism of spread of Cassava brown streak disease (CBSD). Until recently, two distinct potyviruses have been reported to cause the disease. These are Cassava brown streak virus (originated in Tanzania but most widely spread) and Ugandan Cassava brown streak virus (reported in Uga...

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The Symptom and Genetic Diversity of Cassava Brown Streak Viruses Infecting Cassava in East Africa

The genetic and symptom diversity of six virus isolates causing cassava brown streak disease (CBSD) in the endemic (Kenya, Mozambique, and Tanzania) and the recently affected epidemic areas (Uganda) of eastern Africa was studied. Five cassava varieties; Albert, Colombian, Ebwanateraka, TMS60444 (all susceptible) and Kiroba (tolerant) were graft inoculated with each isolate. Based on a number of...

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High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants.

AIMS To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. METHODS AND RESULTS The diagnostic assay was developed for two RNA viruses; Cassava brown streak virus (CBSV) and Uganda cassava brown streak virus (UCBSV) and two predominant DNA viruses; Afr...

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Field Level RNAi-Mediated Resistance to Cassava Brown Streak Disease across Multiple Cropping Cycles and Diverse East African Agro-Ecological Locations

Cassava brown streak disease (CBSD) presents a serious threat to cassava production in East and Central Africa. Currently, no cultivars with high levels of resistance to CBSD are available to farmers. Transgenic RNAi technology was employed to combat CBSD by fusing coat protein (CP) sequences from Ugandan cassava brown streak virus (UCBSV) and Cassava brown streak virus (CBSV) to create an inve...

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Analyses of Twelve New Whole Genome Sequences of Cassava Brown Streak Viruses and Ugandan Cassava Brown Streak Viruses from East Africa: Diversity, Supercomputing and Evidence for Further Speciation

Cassava brown streak disease is caused by two devastating viruses, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) which are frequently found infecting cassava, one of sub-Saharan Africa's most important staple food crops. Each year these viruses cause losses of up to $100 million USD and can leave entire families without their primary food source, for an entire...

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تاریخ انتشار 2013