Recombinant Human Interferon-? Inhibits Adenovirus Multiplication
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چکیده
The susceptibility of adenovirus to the inhibitory effect of human interferons in vitro was investigated. We tested recombinant human interferons-c~2, -/~1 and -7 against adenovirus serotypes 1 and 5 (group C), 3 and 7a (group B), and a wild strain isolated from an acutely ill child who later died. Pretreatment of WISH cells with interferon-? for 24 h induced a dose-dependent inhibition of multiplication of all adenovirus strains tested, the TCIDs0 varying from 25 to 90 IU/ml. Human interferon-c~ 2 was unable to decrease adenovirus multiplication, while interferon-/~l at 2000 IU/ml slightly lowered the yield of adenovirus. Similar results were obtained in HEp-2 and FS-4 cells, while A549 and peripheral blood mononuclear cells were insensitive to interferon-?. The difference between the effects of interferon-? and interferon-c~ and -,8 on adenovirus multiplication in vitro suggests that its mechanism of antiviral action is different.
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