Fall 2000-vs4.pub

Cryopreservation of rat precision-cut liver and kidney slices by rapid freezing and vitrification.

Precision-cut tissue slices of both hepatic and extra-hepatic origin are extensively used as an in vitro model to predict in vivo drug metabolism and toxicity. Cryopreservation would greatly facilitate their use. In the present study, we aimed to improve (1) rapid freezing and warming (200 degrees C/min) using 18% Me(2)SO as cryoprotectant and (2) vitrification with high molarity mixtures of cr...

Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols.

OBJECTIVE To study the thermal properties of a cryoprotectant solution, called VS4, and of VS4-impregnated whole sheep ovaries with pedicle. DESIGN Physical and experimental animal study. SETTING Academic research environment. ANIMAL(S) Five- to 6-month-old ewes. INTERVENTION(S) Thermal properties on cooling of a cryoprotectant solution called VS4 were measured by differential scanning ...

Fall 2007.pub

Overall mortality among patients surviving an episode of peptic ulcer bleeding.

STUDY OBJECTIVE The authors investigated whether patients who have survived an acute episode of peptic ulcer bleeding (PUB) have an excess long term all cause mortality compared with the general population free of PUB. DESIGN Follow up study of previously identified cohort of patients with a PUB episode and a general population cohort. SETTING The source population included all people aged ...

The major outer membrane protein of a single Chlamydia trachomatis serovar can possess more than one serovar-specific epitope.

The major outer membrane proteins (MOMPs) of human Chlamydia trachomatis serovars exhibit four regions of variable amino acid sequences (VS1 to VS4) harboring serovar-specific B-cell epitopes. Antibody responses to these epitopes may contribute to acquired protection against human chlamydial infection. MOMP B-cell epitopes defined by 22 different serovar-specific or bispecific murine monoclonal...