Optimization of Mannanase Production from Streptomyces Sp. Pg-08-03 in Submerged Fermentation
نویسندگان
چکیده
Streptomyces sp. PG-08-3 was isolated from the desert of Rajasthan (India). The organism produced mannanase (15 Umg protein) in the presence of 0.5% guar gum as a sole carbon source in minimal media by submerged fermentation (SmF). Enzyme production was enhanced by 7.3-fold when 0.5% soyabean meal and 0.25% of leucine were added to the minimal media. Increasing the guar gum concentration in the media by 0.1-1.0% resulted in linearly enhanced the production of mannanase.
منابع مشابه
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation
The objective of this study was to investigate the effect of media compositions on the production of βmannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) s...
متن کاملEvaluation of Ca-Independent a-Amylase Production by Bacillus sp. KR-8104 in Submerged and Solid State Fermentation Systems
This study investigates the production of crude Ca-independent and low pH active α-amylase by Bacillussp. KR-8104 in submerged fermentation (SmF) and solid-state fermentation (SSF) systems. Differentparameters were evaluated in each system using “one factor at a time” approach to improve the production ofenzyme. The results showed that in the SmF the maximum enzyme production ...
متن کاملPRODUCTION OF CELLULOSE-DEGRADING ENZYME ON SISAL AND OTHER AGRO-INDUSTRIAL RESIDUES USING A NEW BRAZILIAN ACTINOBACTERIA STRAIN Streptomyces sp. SLBA-08
Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentat...
متن کاملStudy of L-Asparaginase Production by Streptomyces noursei MTCC 10469, Isolated from Marine Sponge Callyspongia diffusa
L-asparaginase is an anti-neoplastic agent used in the chemotherapy of lymphoblastic leukaemia. The present work deals with production of extra-cellular Lasparaginase from marine actinomycetes, using submerged fermentation. Marine actinomycete Streptomyces associated with marine spongeCallyspongia diffusa was isolated using specific ISP medium. Sponge-associated Streptomyces was character...
متن کاملStudy on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation
Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...
متن کامل